Knockout phase
From the last 16 until the semi-finals, clubs play two matches against each other on a home and away basis with the same rules as the qualifying and play-off rounds applied. In the last 16, group winners play runners-up other than teams from their own pool or nation, while from the quarter-finals on the draw is free.
09 March 2010
| Home | Score | Away | |
| Arsenal | 5-0 | Porto | Watch video |
| Fiorentina | 3-2 | Bayern | Watch video |
10 March 2010
| Home | Score | Away | |
| Real Madrid | 1-1 | Lyon | Watch video |
| Manchester United | 4-0 | Milan | Watch video |
16 March 2010
| Home | Score | Away | |
| Sevilla | 20:45 | CSKA | Watch video |
| Chelsea | 20:45 | Inter | Watch video |
17 March 2010
| Home | Score | Away | |
| Barcelona | 20:45 | Stuttgart | Watch video |
| Bordeaux | 20:45 | Olympiacos | Watch video |
